ACQUITY UPLC 顆粒技術(shù)
UPLC®顆粒不同于常規(guī)HPLC 填料顆粒，它需要經(jīng)過(guò)UPLC測(cè)試和認(rèn)證并且能夠耐受UPLC操作壓力（如 15000 psi/1000 bar）。為什么顆粒的耐壓性能如此重要? 為了實(shí)現(xiàn)亞2 µm顆粒的高柱效，需要使用更高的線速度（即更高流速），這種情況下會(huì)產(chǎn)生更高的操作壓力。沃特世公司已經(jīng)推出了兩種高效耐壓的UPLC 顆粒：
1.7 µm亞乙基橋雜化（BEH）顆粒
1.8 µm高強(qiáng)度硅膠（HSS）顆粒
首先推出的是1.7 µm ACQUITY UPLC® BEH 顆粒。它采用第二代雜化顆粒技術(shù)，是UPLC 技術(shù)背后的核心之一，包括五種色譜柱化學(xué)品：
C18
C8
Shield RP18
苯基
HILIC
由于它是雜化顆粒，更寬的pH 值適用范圍（pH 1-12） 使得方法開發(fā)快速簡(jiǎn)便。BEH顆粒也有HPLC 顆粒尺寸（2.5, 3.5, 5 和 10 µm），用在XBridge™ 系列HPLC色譜柱上，這樣可以達(dá)到從HPLC 到UPLC分離方法無(wú)縫轉(zhuǎn)換。
第二種是沃特世公司推出的1.8µm的ACQUITY UPLC HSS（高強(qiáng)度硅膠）顆粒。隨著更多的分離科學(xué)家意識(shí)到超高效液相色譜UPLC技術(shù)在不同應(yīng)用領(lǐng)域的優(yōu)勢(shì)，沃特世公司繼續(xù)生產(chǎn)更多的ACQUITY UPLC 顆粒和化學(xué)品解決方案來(lái)滿足不同的應(yīng)用需求。沃特世公司的材料科學(xué)家開發(fā)了高強(qiáng)度硅膠（HSS） 顆粒，具有高機(jī)械穩(wěn)定性和恰當(dāng)?shù)男螒B(tài)，以滿足長(zhǎng)柱壽命和超高效液相色譜UPLC在高至15000 psi （1000 bar）的壓力下的工作效率。這種1.8 µm微米的超高效液相色譜 UPLC高強(qiáng)度硅膠 HSS 顆粒為UPLC分離而設(shè)計(jì)并經(jīng)特別測(cè)試，包括兩種色譜柱化學(xué)品。
T3 C18
為保留和分離極性有機(jī)化合物而設(shè)計(jì)，低鍵合密度反相C18 柱，100%純水相流動(dòng)相兼容，與Atlantis® T3 HPLC高效液相色譜柱類似。 的ACQUITY UPLC 鍵合相，ACQUITY UPLC HSS C18 色譜柱為堿性化合物的分離提供的峰形，與ACQUITY UPLC BEH C18 色譜柱相比，有教強(qiáng)的保留，并且在低pH值具有相當(dāng)長(zhǎng)的色譜柱壽命。
| 沃特世公司是生產(chǎn)兩種UPLC顆粒的生產(chǎn)商 | ||
| 鍵合相 | 貨號(hào) | 描述 |
| C18 | 186002344 | ACQUITY UPLC BEH C18 1.7UM 1.0X50MM COL |
| 186002346 | ACQUITY UPLC BEH C18 1.7UM 1.0X100MM COL | |
| 186002347 | ACQUITY UPLC BEH C18 1.7UM新型1.0X150MM COL | |
| 186002350 | ACQUITY UPLC BEH C18 1.7UM 2.1X50MM COL | |
| 186002352 | ACQUITY UPLC BEH C18 1.7UM 2.1X100MM COL | |
| 186002353 | ACQUITY UPLC BEH C18 1.7UM 新型2.1X150MM COL | |
| Shield RP18 適用于堿性化合物 | 186002851 | ACQUITY BEH SHIELD RP18 1.7UM 1.0X50MM |
| 186002852 | ACQUITY BEH SHIELD RP18 1.7UM 1.0X100MM | |
| 186003373 | ACQUITY BEH SHIELD RP18 1.7UM 新型1.0X150MM | |
| 186002853 | ACQUITY BEH SHIELD RP18 1.7UM 2.1X50MM | |
| 186002854 | ACQUITY BEH SHIELD RP18 1.7UM 2.1X100MM | |
| 186003376 | ACQUITY BEH SHIELD RP18 1.7UM新型2.1X1500MM | |
| HSS T3 | 186003538 | ACQUITY UPLC HSS T3 1.8µm 2.1x50mm |
| 適用于極性化合物 | 186003539 | ACQUITY UPLC HSS T3 1.8µm 2.1x100mm |
| C8 | 186002875 | ACQUITY UPLC BEH C8 1.7UM 1.0X50MM COL |
| 186002876 | ACQUITY UPLC BEH C8 1.7UM 1.0X100MM COL | |
| 186003374 | ACQUITY UPLC BEH C8 1.7UM新型1.0X150MM COL | |
| 186002877 | ACQUITY UPLC BEH C8 1.7UM 2.1X50MM COL | |
| 186002878 | ACQUITY UPLC BEH C8 1.7UM 2.1X100MM COL | |
| 186003377 | ACQUITY UPLC BEH C8 1.7UM 新型2.1X150MM COL | |
| 苯基柱 | 186002882 | ACQUITY UPLC BEH PHENYL 1.7UM 1.0X50MM |
| 186002883 | ACQUITY UPLC BEH PHENYL 1.7UM 1.0X100MM | |
| 186003378 | ACQUITY UPLC BEH PHENYL 1.7UM新型1.0X150MM | |
| 186002884 | ACQUITY UPLC BEH PHENYL 1.7UM 2.1X50MM | |
| 186002885 | ACQUITY UPLC BEH PHENYL 1.7UM 2.1X100MM | |
| 186003378 | ACQUITY UPLC BEH PHENYL 1.7UM 新型2.1X150MM | |
| HILIC | 186003457 | ACQUITY UPLC BEH HILIC 1.7µM 新型1.0 X 50MM |
| 186003458 | ACQUITY UPLC BEH HILIC 1.7µM 新型1.0 X 100MM | |
| 186003459 | ACQUITY UPLC BEH HILIC 1.7µM新型1.0 X 150MM | |
| 186003460 | ACQUITY UPLC BEH HILIC 1.7µM 新型2.1 X 50MM | |
| 186003461 | ACQUITY UPLC BEH HILIC 1.7µM 新型2.1 X 100MM | |
| 186003462 | ACQUITY UPLC BEH HILIC 1.7µM 新型2.1 X 150MM | |
| ACQUITY UPLC色譜柱在線過(guò)濾系統(tǒng) | ||
| 貨號(hào) | 描述 | |
| 205000343 | 在線過(guò)濾卡套和六個(gè)0.2um不銹鋼過(guò)濾器 | |
| 700002775 | 適用于205000343的五個(gè)0.2um不銹鋼替換過(guò)濾芯 | |
| ACQUITY UPLC反相色譜柱4根/包 | ||
| 貨號(hào) | 尺寸 | 描述 |
| 176001042 | 2.1 X50mm | 所有四種反相色譜柱 |
| 176001043 | 2.1 X100mm | 所有四種反相色譜柱end--> |
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